Plant Transcription Factor Database
Previous version: v3.0
Populus euphratica
FAR1 Family
Species TF ID Description
CCG000527.3FAR1 family protein
CCG003134.1FAR1 family protein
CCG004426.1FAR1 family protein
CCG005265.1FAR1 family protein
CCG005564.1FAR1 family protein
CCG006034.1FAR1 family protein
CCG006967.1FAR1 family protein
CCG007207.1FAR1 family protein
CCG008098.1FAR1 family protein
CCG009166.2FAR1 family protein
CCG009636.1FAR1 family protein
CCG011494.1FAR1 family protein
CCG014036.1FAR1 family protein
CCG015330.1FAR1 family protein
CCG016991.4FAR1 family protein
CCG019286.1FAR1 family protein
CCG019911.1FAR1 family protein
CCG021402.1FAR1 family protein
CCG022113.1FAR1 family protein
CCG025002.1FAR1 family protein
CCG025862.1FAR1 family protein
CCG026764.1FAR1 family protein
CCG026765.1FAR1 family protein
CCG027164.2FAR1 family protein
CCG028694.1FAR1 family protein
CCG029774.2FAR1 family protein
CCG033707.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885